Xu Wenming
West China Second University Hospital, China
Title: Dysfunction of miRNA biogenesis machinary proteins between adipose and ovary tissue contribute to PCOS etiology
Biography
Biography: Xu Wenming
Abstract
miRNAs are important regulators of glucose metabolism and oxidative stress, which play key roles in the pathogenesis of PCOS. The objective was to study the expression of miRNA biogenesis related genes, including Dicer1, Ago2 and miR-15b and miR-503 in adipose of PCOS and normal controls, and further investigate the relationship of dysregulation of miRNA level with key parameters related to insulin resistance and oxidative stress in tissue level. PCOS patients and normal controls submitted to laparoscopic surgery were included, and adipose tissue and serum samples were collected. Our result shows that significantly reduced expression of Dicer1 were detected in adipose of PCOS patients. Correlation analysis indicated that miR-15b expression is positively correlated with Glut4 expression, while negatively correlatived with T-SOD. Our study also shows that follicular fluid contains microvesicle, which express Dicer proteins and could mediate the effects on phenotype of cumulus cells in ovary. Our result shows that dysregulation of miRNA biogenesis is related to the adipose insulin resistance and oxidative stress, and miR-15/16 famlily members, such as miR-15b is critical important for the insulin resistance and oxidative stress through targeting Glut4 pathway, thus represent a promising target of PCOS diagnosis and therapy.