Qin Lang
West China Second University Hospital, China
Title: Differential Expression Proï¬le of Immunological Cytokines in Local Ovary in Patients with Polycystic Ovarian Syndrome: analysis by Flow Cytometry
Biography
Biography: Qin Lang
Abstract
Objective: Immune dysregulation may play an important role in the pathogenesis of polycystic ovary syndrome (PCOS). The purpose of this study was to investigate the Th1 and Th2-related cytokine proï¬le in local ovary of women with PCOS.
Study Design: The T lymphocytes of follicular fluid (FF) were obtained at the time of oocyte retrieval before in-vitro fertilization (IVF) in woman with or without PCOS. After culturing with PMA, Ionomycin and Golgi stop agent, cells were detected for the intracellular cytokine production by flow cytometry. The proï¬le of Th1 (IFN-g, IL-2) and Th2 (IL-4, IL-10) cytokines of CD3+CD4+ T lymphocyte subsets were analyzed through invert gating. These cytokines in FF were also evaluated by ELISA.
Results: Flow cytometry analysis showed that the production of Th1 (IFN-g, IL-2) cytokines in FF lymphocytes in PCOS patients were signiï¬cantly higher than those in controls; ELISA result also demonstrated that the concentration of Th1 cytokines (IFN-γ, IL-2) in FF in PCOS patients is signiï¬cantly increased compared with those in controls.
Conclusion: It is concluded that the immune dominance of Th1 may be the immunological feature of the ovary in PCOS patients. It might participate in the immune pathogenesis in the ovary of PCOS patients. These results suggest that chronic inflammation maybe one of the underlying mechanism for the pathogenesis of PCOS